Iron assay kit (Nitroso-PSAP)

Iron is a mineral (functioning as an enzyme cofactor) that plays an essential role in many biological processes. It is essential to nearly all known organisms. As a transition element it can form a range of oxidation states, the most common being Fe²⁺ (or ferrous iron) and Fe³⁺ (or ferric iron). Ingested iron is mainly absorbed in the form of Fe²⁺. The trivalent form and the heme-bound Fe²⁺-component of iron is reduced by vitamin C. Before passing into the plasma, it is oxidized by ceruloplasmin to Fe³⁺ and bound to transferrin to form a transferrin-iron complex. Iron is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions. Iron is important for redox reactions, oxygen transport (e.g. hemoglobin), short-term oxygen storage (e.g. myoglobin) and energy generation. Iron deficiency has many adverse consequences, including anemia, hemochromatosis, chronic renal disease and in children, behavioral and learning disorders. Iron excess is toxic to the body, harming the heart, liver, skin, pancreatic islet beta cells, bones, joints, and pituitary gland. Maintaining proper iron balance is essential for maintaining homeostasis and health.

Features

• Using microplate reader for assay, no need to use Atomic Absorption Spectrometry or ICP-OES Optical Emission Spectrometry, which have been mainly used so far.
• Good correlation with the result of Atomic Absorption Spectrometry and ICP-OES.
• High throughput measurement by using microplate reader.
• Assay range: 10-1,000 μg/dL
• All animal species sample can be measured.
• Assay time : just 10 minutes!
• 1 point calibration – do not need multiple point measurement to make standard curve (r^2=0.9999).
• Kit does not contain any hazardous components like cyanide or azide as preservative.
• Samples : Serum, Plasma, Urine, Saliva, Cell lysate, Tissue extract, Hair extract, Water, etc
• In combination with UIBC Assay kit LS Bathophenanthroline method (#UIB01E), TIBC (Total Iron Binding Capacity) can be measured.

Assay Principle