ISG15 Antibody| F41498-0.08ML/F41498-0.4ML/R32374/RQ5108

ISG15 Antibody| F41498-0.08ML/F41498-0.4ML/R32374/RQ5108 | Gentaur UK, US & Europe Distribution

Background: ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: N/A

Application: WB, IHC, ELISA

Application Details: IHC (Paraffin): 1:50-1:100, Western blot: 1:1000

Application Note: Titration of the ISG15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This ISG15 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 31-61 from the human protein was used as the immunogen for this ISG15 antibody.

Storage: Aliquot the ISG15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A