K11-linked Di-Ubiquitin | 6421

Increasingly, researchers are focusing on the role poly-ubiquitin chains linked through K11. This post-translational modification has been linked to the ERAD cycle as a signal, similar to K48 linkage, for proteasomal degradation. More recently, K11 linkage appears to play an important role in cell cycle signaling, as it is associated with the anaphase promoting complex (APC) of ubiquitination machinery. These di-ubiquitin chains are generated from the enzymatic linkage (UBE2S) of wild-type ubiquitin through lysine 11 and purified to >95% homogeneity by ion exchange chromatography.

6421 | K11-linked Di-Ubiquitin DataSheet

Biomolecule/Target: N/A

Synonyms: K11-linked Di-Ubiquitin

Alternates names: N/A

Taglines: Substrate for defined length polyubiquitination

NCBI Gene ID #: 20296

NCBI Gene Symbol: MCP-1

Gene Source: N/A

Accession #: P10148

Recombinant: N/A

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: Western Blot

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by measuring the dose dependent chemotaxis with human THP-1 cells. The ED is in the range of 1-10 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.111 kDa (Band migrates faster on Gels)

Concentration: 2.5 mg/ml

Appearance: Liquid

Physical form description: 2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A