Description
Poly-ubiquitylation of target proteins through linkage at K48, is now the most thoroughly studied of the various chain linkages, and was once considered the hallmark of this post-translational modification. It is now clear that many, if not all, poly-Ub chain topologies likely play distinct and important roles in regulating cellular processes. Nevertheless, K48 linkage remains a critical pathway for the cells to maintain homeostasis through proteolytic degradation, and as such remains very intriguing for the study of DUBs that play a role in the degradation, as well as the proteasome itself. These di-ubiquitin chains are generated from the enzymatic linkage (E2-25K) of wild-type ubiquitin through lysine 48. The most distal ubiquitin contains an arginine substitution for a lysine at position 48, limiting the chain length.
6415 | K48-linked Di-Ubiquitin DataSheet
Biomolecule/Target: N/A
Synonyms: K48-linked Di-Ubiquitin
Alternates names: N/A
Taglines: Substrate for defined length polyubiquitination
NCBI Gene ID #: 14825
NCBI Gene Symbol: GROA
Gene Source: N/A
Accession #: P12850
Recombinant: N/A
Source: N/A
Purity by SDS-PAGEs: 98%
Assay: Western Blot
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by its ability to chemoattract total human neutrophils population using a concentration range of 10-100 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 17.139 kDa (Band migrates faster on Gels)
Concentration: 2.5 mg/ml
Appearance: Liquid
Physical form description: 2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can be diluted into other aqueous buffers
Amino acid sequence: N/A