K48-linked Tetra-Ubiquitin | 6417

Poly-ubiquitylation of target proteins through linkage at K48, is now the most thoroughly studied of the various chain linkages, and was once considered the hallmark of this post-translational modification. It is now clear that many, if not all, poly-Ub chain topologies likely play distinct and important roles in regulating cellular processes. Nevertheless, K48 linkage remains a critical pathway for the cells to maintain homeostasis through proteolytic degradation, and as such remains very intriguing for the study of DUBs that play a role in the degradation, as well as the proteasome itself. These tetra-ubiquitin chains are generated from the enzymatic linkage (E2-25K) of wild-type ubiquitin through lysine 48. The most distal ubiquitin contains an arginine substitution for a lysine at position 48, limiting the chain length.

6417 | K48-linked Tetra-Ubiquitin DataSheet

Biomolecule/Target: N/A

Synonyms: K48-linked Tetra-Ubiquitin

Alternates names: N/A

Taglines: Substrate for defined length polyubiquitination

NCBI Gene ID #: 3479

NCBI Gene Symbol: IGF1

Gene Source: N/A

Accession #: P01343

Recombinant: N/A

Source: N/A

Purity by SDS-PAGEs: 97%

Assay: Western Blot

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by a cell proliferation assay using NIH3T3 cells is typically 1-6 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 34.233 kDa (Band migrates faster on Gels)

Concentration: 2.5 mg/ml

Appearance: Liquid

Physical form description: 2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.

Reconstitution Instructions: Reconstitute in dHO or 10 mM AcOH to 1.0 mg/ml. This solution can then be diluted into other buffered solutions or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A