K63-linked Di-Ubiquitin | 6418

Poly-ubiquitination of target proteins through K63 has recently become the focus of intense study. The topology of this linkage type is quite different from polyubiquitin linked through lysine 48. Modification of proteins by K63-linked polyubiquitination has been implicated in, among other cellular processes, the regulation of the DNA damage response, endosomal sorting, autophagy of misfolded/aggregated proteins, and neurodegeneration. These di-ubiquitin chains are generated from the enzymatic linkage of wild-type ubiquitin through lysine 63. The most distal ubiquitin contains an arginine substitution for a lysine at position 63, limiting chain length.

6418 | K63-linked Di-Ubiquitin DataSheet

Biomolecule/Target: N/A

Synonyms: K63-linked Di-Ubiquitin

Alternates names: N/A

Taglines: Substrate for defined length polyubiquitination

NCBI Gene ID #: 3479

NCBI Gene Symbol: IGF1

Gene Source: N/A

Accession #: P01343

Recombinant: N/A

Source: N/A

Purity by SDS-PAGEs: 97%

Assay: Western Blot

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by a cell proliferation assay using NIH3T3 cells is typically 1-6 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.139 kDa (Band migrates faster on Gels)

Concentration: 2.5 mg/ml

Appearance: Liquid

Physical form description: 2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.

Reconstitution Instructions: Reconstitute in dHO or 10 mM AcOH to 1.0 mg/ml. This solution can then be diluted into other buffered solutions or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A