LEF1 Antibody| F41886-0.08ML/F41886-0.4ML/F53737-0.1ML/R35290-100UG

LEF1 Antibody| F41886-0.08ML/F41886-0.4ML/F53737-0.1ML/R35290-100UG | Gentaur UK, US & Europe Distribution

Background: Lymphoid enhancer-binding factor 1 participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. [UniProt]

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: Mouse, Rat

Application: WB, IF, ELISA

Application Details: Western blot: 1:1000, Immunofluorescence: 1:10-1:50

Application Note: Titration of the LEF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This LEF1 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 10-37 from the human protein was used as the immunogen for this LEF1 antibody.

Storage: Aliquot the LEF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A