LIN28B Antibody| F44235-0.08ML/F44235-0.4ML/F44237-0.05ML/F44237-0.2ML

LIN28B Antibody| F44235-0.08ML/F44235-0.4ML/F44237-0.05ML/F44237-0.2ML | Gentaur UK, US & Europe Distribution

Background: Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.

Format: Purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: N/A

Application: IF, WB, IHC, FACS, ELISA

Application Details: Immunofluorescence: 1:100, Western blot: 1:1000, IHC (Paraffin): 1:10-1:50, Flow Cytometry: 1:10-1:50

Application Note: Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This LIN28B antibody is available for research use only.

Purity: Purified

Immunogen: A portion of amino acids 1-30 from the human protein was used as the immunogen for this LIN28B antibody.

Storage: Aliquot the LIN28B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A