26
MMP-9, Active, Human Recombinant | P1567
- SKU:
- 26-P1567-GEN
- Availability:
- Usually shipped in 5 working days
Description
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B, is the mostly studied MMP, due to its fundamental role in cancer biology, autoimmune disease, and other conditions. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is structurally a multi-domain metalloenzyme, composed of a prodomain, a catalytic domain, a gelatin binding domain, a metal-binding domain, and a carboxyl terminal hemopexin like domain. This active human MMP-9 is composed of the catalytic domain, a gelatin binding domain, and a metal-binding domain (AA 107-457). The protein was expressed in E. coli and purified and refolded using proprietary techniques.
P1567 | MMP-9, Active, Human Recombinant DataSheet
Biomolecule/Target :
Synonyms: Matrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2
Alternates names: Matrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2
Taglines: A Protein involved in breakdown of extracellular matrix
NCBI Gene ID #: 4319
NCBI Gene Symbol: MMP-9
Gene Source: Human
Accession #: P14780
Recombinant: TRUE
Source: E. coli
Purity by SDS-PAGE: >95%
Assay: SDS - PAGE
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): The activity was determined using fluorogenic substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is ≥ 200 uU/ug.
Biological activity:
Results:
Binding Capacity:
Unit Definition: One unit (U) of the activity is defined as the amount of enzyme that releases 1 umole of Mca from Mca-PLGL-Dpa-AR-NH2 per min. at 37 °C.
Molecular Weight: 41.3 KDa (N-terminal His-Tag)
Concentration:
Appearance: Lyophilized
Physical form description: Lyophilized from a proprietary buffer
Reconstitution Instructions: Reconstitute with pre-chilled 30% Glycerol/H2O solution to 0.1 ug/ul and incubate on ice until it completely dissolves. Aliquot and store the reconstituted MMP-9 at -20°C. Stable for 2 months after reconstitution.
Amino acid sequence: aa 107-457
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
Additional Information
Storage Temperature: |
-20°C |
Shipping: |
Gel Pack |
Shelf Life: |
6 months |