MMP-9, Active, Human Recombinant | P1567

Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B, is the mostly studied MMP, due to its fundamental role in cancer biology, autoimmune disease, and other conditions. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is structurally a multi-domain metalloenzyme, composed of a prodomain, a catalytic domain, a gelatin binding domain, a metal-binding domain, and a carboxyl terminal hemopexin like domain. This active human MMP-9 is composed of the catalytic domain, a gelatin binding domain, and a metal-binding domain (AA 107-457). The protein was expressed in E. coli and purified and refolded using proprietary techniques.

P1567 | MMP-9, Active, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Matrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2

Alternates names: Matrix Metalloproteinase-9, Gelatinase B, 92 kDa Type IV Collagenase, MMP9, CLG4B, GELB, MANDP2

Taglines: A Protein involved in breakdown of extracellular matrix

NCBI Gene ID #: 4319

NCBI Gene Symbol: MMP-9

Gene Source: Human

Accession #: P14780

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): The activity was determined using fluorogenic substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is ≥ 200 uU/ug.

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit (U) of the activity is defined as the amount of enzyme that releases 1 umole of Mca from Mca-PLGL-Dpa-AR-NH2 per min. at 37 °C.

Molecular Weight: 41.3 KDa (N-terminal His-Tag)

Concentration:

Appearance: Lyophilized

Physical form description: Lyophilized from a proprietary buffer

Reconstitution Instructions: Reconstitute with pre-chilled 30% Glycerol/H2O solution to 0.1 ug/ul and incubate on ice until it completely dissolves. Aliquot and store the reconstituted MMP-9 at -20°C. Stable for 2 months after reconstitution.

Amino acid sequence: aa 107-457

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans