Monoglyceride lipase, human recombinant | P1102

MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

P1102 | Monoglyceride lipase human recombinant DataSheet

Biomolecule/Target: MGLL

Synonyms: MLL, HU-K5, HUK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like

Alternates names: MLL, HU-K5, HUK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like

Taglines: MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

NCBI Gene ID #: 20302

NCBI Gene Symbol: CCL3

Gene Source: Human

Accession #: P10855

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at 25C.

Biological activity: Determined by its ability to chemoattract Balb/c peripheral blood MNCs and mouse Balb/c splenocytes using a concentration range of 10-100 ng/ml.

Results: >170 units/mg,

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at 25C.

Molecular Weight: 36.4 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A