26
Monoglyceride lipase, human recombinant | P1102
- SKU:
- 26-P1102-GEN
- Availability:
- Usually shipped in 5 working days
Description
MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
P1102 | Monoglyceride lipase human recombinant DataSheet
Biomolecule/Target: MGLL
Synonyms: MLL, HU-K5, HUK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like
Alternates names: MLL, HU-K5, HUK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like
Taglines: MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
NCBI Gene ID #: 20302
NCBI Gene Symbol: CCL3
Gene Source: Human
Accession #: P10855
Recombinant: Yes
Source: E. Coli
Purity by SDS-PAGEs: 99%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at 25C.
Biological activity: Determined by its ability to chemoattract Balb/c peripheral blood MNCs and mouse Balb/c splenocytes using a concentration range of 10-100 ng/ml.
Results: >170 units/mg,
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at 25C.
Molecular Weight: 36.4 kDa
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A