Mouse 4-Hydroxynonenal (HNE) ELISA Kit | ELK8374

Mouse 4-Hydroxynonenal (HNE) ELISA Kit | ELK8374 | Gentaur UK, US & Europe Distribution

Alternative Names: 4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal

Species: Mouse

Assay Type: Sandwich

Sensitivity: 1.12 ng/mL

Standard: 150 ng/mL

Detection Range: 2.35-150 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HNE (4-Hydroxynonenal) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HNE (4-Hydroxynonenal) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HNE (4-Hydroxynonenal), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNE (4-Hydroxynonenal) in the samples is then determined by comparing the OD of the samples to the standard curve.