Description
NCBP1 Antibody | 7395 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Chicken: (81%)
Immunogen: NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1.
The immunogen is located within amino acids 420 - 470 of NCBP1.
Research Area: Homeostasis
Tested Application: E, WB, ICC
Application: NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificiy: NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.
Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 87 kDa
Observed: 87 kDa
Validation: N/A
Isoform: N/A
Purification: NCBP1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: NCBP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: NCBP1 Antibody: NCBP, Sto1, CBP80, NCBP, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC) , which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1, 2) . Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3) .