NCBP1 Antibody | 7395

NCBP1 Antibody | 7395 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (81%)

Immunogen: NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1.
The immunogen is located within amino acids 420 - 470 of NCBP1.

Research Area: Homeostasis

Tested Application: E, WB, ICC

Application: NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Specificiy: NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 87 kDa
Observed: 87 kDa

Validation: N/A

Isoform: N/A

Purification: NCBP1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NCBP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: NCBP1 Antibody: NCBP, Sto1, CBP80, NCBP, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC) , which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1, 2) . Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3) .