NDST2 Antibody | 7869

NDST2 Antibody | 7869 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: NDST2 antibody was raised against a 16 amino acid peptide near the amino terminus of human NDST2.
The immunogen is located within amino acids 20 - 70 of NDST2.

Research Area: Stem Cell

Tested Application: E, WB, IF

Application: NDST2 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: NDST2 antibody is human, mouse and rat reactive. At least two isoforms of NDST2 are known to exist; this antibody will only detect the larger isoform. NDST2 antibody is predicted not to cross-react with other members of the NDST protein family.

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 97 kDa
Observed: 101 kDa

Validation: N/A

Isoform: N/A

Purification: NDST2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NDST2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NDST2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: NDST2 Antibody: NST2, HSST2, N-heparan sulfate sulfotransferase 2, NDST-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2) protein, also known as N-heparan sulfate sulfotransferase 2, and the highly related protein NDST1 have key roles in the sulfation of heparan sulfate proteoglycans, molecules that are present of cell surfaces and the extracellular matrix (1, 2) . NDST1 and NDST2 are dispensable for mesodermal differentiation into osteoblasts but necessary for induction of adipocytes and neural cells (3) .