Description
NFYB Antibody | 22-843 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1) .
Research Area: Signal Transduction, Transcription
Tested Application: WB, IF
Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Specificiy: N/A
Positive Control 1: HL-60
Positive Control 2: SKOV3
Positive Control 3: HeLa
Positive Control 4: Mouse liver
Positive Control 5: Mouse spleen
Positive Control 6: Mouse brain
Molecular Weight: Observed: 28-30kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: NFYB, HAP3, CBF-A, CBF-B, NF-YB
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.