NFYB Antibody | 22-843

NFYB Antibody | 22-843 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1) .

Research Area: Signal Transduction, Transcription

Tested Application: WB, IF

Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:100

Specificiy: N/A

Positive Control 1: HL-60

Positive Control 2: SKOV3

Positive Control 3: HeLa

Positive Control 4: Mouse liver

Positive Control 5: Mouse spleen

Positive Control 6: Mouse brain

Molecular Weight: Observed: 28-30kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: NFYB, HAP3, CBF-A, CBF-B, NF-YB

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.