OryzaExp™ Epidermal Growth Factor, Human Recombinant | P1398

Human epidermal growth factor, also known as EGF, is a 6kD protein with 53 amino acid residues and three intramolecular disulfide bonds. By binding with high affinity to epidermal growth fact receptor (EGFR) on the cell surface, EGF results in a variety of biochemical changes within the cell, rising intracellular calcium levels, increasing glycolysis, protein synthesis and regulating the gene expression, including the gene for EGFR that ultimately lead to DNA synthesis and cell proliferation.

P1398 | OryzaExp Epidermal Growth Factor Human Recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Recombinant epidermal growth factor, Plant-derived EGF, recombinant EGF, rEGF, EGF

Alternates names: Recombinant epidermal growth factor, Plant-derived EGF, recombinant EGF, rEGF, EGF

Taglines: A growth factor stimulating DNA synthesis, cell growth and proliferation by binding to EGFR

NCBI Gene ID #: 1839

NCBI Gene Symbol: HBEGF

Gene Source: Human

Accession #: Q99075

Recombinant: False

Source: Oryza Sativa (Rice)

Purity by SDS-PAGEs: 97%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: 5EU/mg

Activity (Specifications/test method): The bioactivity determined by a cell proliferation assay using Balb/c 3T3, corresponding to a specific activity of 1×106 Units/mg.

Biological activity: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 units/mg.

Results:

Binding Capacity: N/A

Unit Definition:

Molecular Weight: 5.3KDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: White lyophilized powder

Reconstitution Instructions: It is recommended to reconstitute the lyophilized Human HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Amino acid sequence: DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL.