Description
Mitogen-activated protein kinase (MAPK) cascades represent one of the major signal systems used by eukaryotic cells to transduce extracellular signals into cellular responses, and involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The stress-activated protein kinase 4 (SAPK4), or p38 delta, is a member of the MAPK family that are activated by chemical and environmental stresses as well as by proinflammatory cytokines. SAPK4 has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines (1). MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase (2).
7754 | P38delta Active DataSheet
Biomolecule/Target: N/A
Synonyms: p38, mitogen-activated protein kinase 13
Alternates names: p38, mitogen-activated protein kinase 13
Taglines: Involved in a wide variety of cellular processes
NCBI Gene ID #: 627
NCBI Gene Symbol: BDNF
Gene Source: N/A
Accession #: P23560
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): 259 nmol phosphate incorporated into MBP protein per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 71.0 kDa
Concentration: 5µg/50 µl
Appearance: Liquid
Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100g/ml.). This can further be diluted to other aqueous buffers.
Amino acid sequence: Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.