PEDF, mouse recombinant | 7572

Pigment epithelium-derived factor (PEDF) is a 47 kDa secreted glycoprotein that belongs to the non-inhibitory serpin family group. PEDF is widely expressed in adult and fetal tissues, including brain, spinal cord, plasma, bone, prostate, pancreas, heart and lung. PEDF acts as an angiogenesis inhibitor with neurotrophic, immunomodulation and antitumor properties. It functions as anti-angiogenic agent by counterbalancing the proangiogenic effect of VEGF. PEDF is one of the most abundant proteins released by adipocytes and induces insulin resistance in adipocytes and human skeletal muscle cells. Recently, it has been reported that PEDF is sufficient to maintain the self-renewal of pluripotent human embryonic stem cells.

7572 | PEDF mouse recombinant DataSheet

Biomolecule/Target: PEDF

Synonyms: Pigment Epithelium-derived Factor; Stromal Cell-derived Factor 3; Serpin F1; Caspin

Alternates names: Pigment Epithelium-derived Factor; Stromal Cell-derived Factor 3; Serpin F1; Caspin

Taglines: An angiogenesis inhibitor with neurotrophic, immunomodulation and antitumor properties

NCBI Gene ID #: 3627

NCBI Gene Symbol: CXCL10

Gene Source: Mouse

Accession #: P02778

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <0.1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: The ED50 of rHu CXCL10 as determined by its ability to chemoattract human CXCR3 transfected BaF3 mouse proB cells was 0.02-0.06 g/mL.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~47.0 kDa. Mouse PEDF (aa 20-417) is fused at the C-terminus to a FLAG®-tag.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized in PBS.

Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml). This can further be diluted to other aqueous buffers.

Amino acid sequence: Recombinant human CXCL10/IP-10/CRG-2 is produced by E.coli transformed with a plasmid contains sequence (Val22-Pro98) of human CXCL10/IP-10/CRG-2 (Uniprot Entry: P02778) fused with a polyhistidine tag at the C-terminus. The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Leu-Ser-Arg.