Description
Pigment epithelium-derived factor (PEDF) is a 47 kDa secreted glycoprotein that belongs to the non-inhibitory serpin family group. PEDF is widely expressed in adult and fetal tissues, including brain, spinal cord, plasma, bone, prostate, pancreas, heart and lung. PEDF acts as an angiogenesis inhibitor with neurotrophic, immunomodulation and antitumor properties. It functions as anti-angiogenic agent by counterbalancing the proangiogenic effect of VEGF. PEDF is one of the most abundant proteins released by adipocytes and induces insulin resistance in adipocytes and human skeletal muscle cells. Recently, it has been reported that PEDF is sufficient to maintain the self-renewal of pluripotent human embryonic stem cells.
7572 | PEDF mouse recombinant DataSheet
Biomolecule/Target: PEDF
Synonyms: Pigment Epithelium-derived Factor; Stromal Cell-derived Factor 3; Serpin F1; Caspin
Alternates names: Pigment Epithelium-derived Factor; Stromal Cell-derived Factor 3; Serpin F1; Caspin
Taglines: An angiogenesis inhibitor with neurotrophic, immunomodulation and antitumor properties
NCBI Gene ID #: 3627
NCBI Gene Symbol: CXCL10
Gene Source: Mouse
Accession #: P02778
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <0.1 EU/g by LAL method
Activity (Specifications/test method): N/A
Biological activity: The ED50 of rHu CXCL10 as determined by its ability to chemoattract human CXCR3 transfected BaF3 mouse proB cells was 0.02-0.06 g/mL.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: ~47.0 kDa. Mouse PEDF (aa 20-417) is fused at the C-terminus to a FLAG®-tag.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized in PBS.
Reconstitution Instructions: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 g/ml). This can further be diluted to other aqueous buffers.
Amino acid sequence: Recombinant human CXCL10/IP-10/CRG-2 is produced by E.coli transformed with a plasmid contains sequence (Val22-Pro98) of human CXCL10/IP-10/CRG-2 (Uniprot Entry: P02778) fused with a polyhistidine tag at the C-terminus. The sequence of the first five N-terminal amino acids was determined and was found to be Val-Pro-Leu-Ser-Arg.