Description
PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
7820 | PGK2 human recombinant DataSheet
Biomolecule/Target: PGK2
Synonyms: Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS
Alternates names: Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS
Taglines: Catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate.
NCBI Gene ID #: 9547
NCBI Gene Symbol: CXCL14
Gene Source: Human
Accession #: O95715
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED50 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.
Results: Specific activity is >11 units/mg
Binding Capacity: N/A
Unit Definition: One unit will convert 1.0 µmole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25ºC
Molecular Weight: 46.9 kDa (437 aa, 1-417 aa + His Tag)
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT
Reconstitution Instructions: Reconstitute the lyophilized recombinant human CXCL14 in sterile 18M-cm H2O not less than 100 g/ml. This can further be diluted to other aqueous buffers.
Amino acid sequence: Recombinant Human CXCL14 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 9.4 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys.