PGK2, human recombinant | 7820

PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

7820 | PGK2 human recombinant DataSheet

Biomolecule/Target: PGK2

Synonyms: Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS

Alternates names: Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS

Taglines: Catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate.

NCBI Gene ID #: 9547

NCBI Gene Symbol: CXCL14

Gene Source: Human

Accession #: O95715

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED50 as determined by its ability to induce calcium flux of prostaglandin E2 treated THP1 human acute monocytic leukemia cells was 1.0-10.0 ng/ml.

Results: Specific activity is >11 units/mg

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25ºC

Molecular Weight: 46.9 kDa (437 aa, 1-417 aa + His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT

Reconstitution Instructions: Reconstitute the lyophilized recombinant human CXCL14 in sterile 18M-cm H2O not less than 100 g/ml. This can further be diluted to other aqueous buffers.

Amino acid sequence: Recombinant Human CXCL14 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 9.4 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Lys-Cys-Lys-Cys.