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Phosphoenolpyruvate Carboxykinase (PCK1), Human Recombinant | P1229
- SKU:
- 26-P1229-GEN
- Availability:
- Usually shipped in 5 working days
Description
Phosphoenolpyruvate Carboxykinase (PCK1) belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PCK1, also called PEPCK-C) and mitochondrial form (PEPCK-M). PCK1 is a control point enzyme which catalyzes a irreversible step in gluconeogenesis.
P1229 | Phosphoenolpyruvate Carboxykinase PCK1 Human Recombinant DataSheet
Biomolecule/Target: PCK1
Synonyms: PEPCK, PEPCK-C
Alternates names: IL20, NIRF
Taglines: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP)
NCBI Gene ID #: 27190
NCBI Gene Symbol: IL17B
Gene Source: Human
Accession #: Q9UHF5
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to induce IL-8 production in human PBMCs using a concentration range of 10.0-100.0 ng/ml. Please note: Results may vary with PBMC donors.
Biological activity: Determined by its ability to induce IL-8 production in human PBMCs using a concentration range of 10.0-100.0 ng/ml. Please note: Results may vary with PBMC donors.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 36.6 kDa
Concentration: N/A
Appearance: Lyophilized
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS, pH 7.5.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F