Phosphoenolpyruvate Carboxykinase (PCK1), Human Recombinant | P1229

Phosphoenolpyruvate Carboxykinase (PCK1) belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PCK1, also called PEPCK-C) and mitochondrial form (PEPCK-M). PCK1 is a control point enzyme which catalyzes a irreversible step in gluconeogenesis.

P1229 | Phosphoenolpyruvate Carboxykinase PCK1 Human Recombinant DataSheet

Biomolecule/Target: PCK1

Synonyms: PEPCK, PEPCK-C

Alternates names: IL20, NIRF

Taglines: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP)

NCBI Gene ID #: 27190

NCBI Gene Symbol: IL17B

Gene Source: Human

Accession #: Q9UHF5

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to induce IL-8 production in human PBMCs using a concentration range of 10.0-100.0 ng/ml. Please note: Results may vary with PBMC donors.

Biological activity: Determined by its ability to induce IL-8 production in human PBMCs using a concentration range of 10.0-100.0 ng/ml. Please note: Results may vary with PBMC donors.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 36.6 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS, pH 7.5.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F