Description
PKA C- (PKAcg) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C- and C- and showed tissue-specific expression. Whereas at the amino acid level C- and C- showed 93% homology, C- showed only about 80% homology to both C- and C- (1). The PRKACG gene is intronless, contains remnants of a poly (A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2).
7728 | PKA cg Active DataSheet
Biomolecule/Target: N/A
Synonyms: PKA, cAMP-dependent protein kinase catalytic subunit gamma
Alternates names: PKA, cAMP-dependent protein kinase catalytic subunit gamma
Taglines: Target for the small GTP binding proteins
NCBI Gene ID #: 4803
NCBI Gene Symbol: NGF
Gene Source: N/A
Accession #: P01138
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): The specific activity of PKAc was determined to be 145 nmol /min/mg as per activity assay protocol.
Biological activity: The ED as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 65.0 kDa
Concentration: 0.1µg/ µl
Appearance: Liquid
Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Reconstitution Instructions: Reconstituted human NGF- should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.
Amino acid sequence: N/A