PKA cg, Active | 7728

PKA C- (PKAcg) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C- and C- and showed tissue-specific expression. Whereas at the amino acid level C- and C- showed 93% homology, C- showed only about 80% homology to both C- and C- (1). The PRKACG gene is intronless, contains remnants of a poly (A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2).

7728 | PKA cg Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKA, cAMP-dependent protein kinase catalytic subunit gamma

Alternates names: PKA, cAMP-dependent protein kinase catalytic subunit gamma

Taglines: Target for the small GTP binding proteins

NCBI Gene ID #: 4803

NCBI Gene Symbol: NGF

Gene Source: N/A

Accession #: P01138

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of PKAc was determined to be 145 nmol /min/mg as per activity assay protocol.

Biological activity: The ED as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 65.0 kDa

Concentration: 0.1µg/ µl

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: Reconstituted human NGF- should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid freeze/thaw cycles.

Amino acid sequence: N/A