PKC gamma, Active | 7764

PKC is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. In the brain, PKC is translocated to cell membranes during ischemia and is rapidly removed or degraded during the second otherwise lethal ischemic insult in preconditioned brains. This suggest that ischemic preconditioning enhances downregulation of cell signaling mediated by PKC and may thereby provide neuroprotection (1).

7764 | PKC gamma Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKC, protein kinase C, gammaprotein kinase C

Alternates names: PKC, protein kinase C, gammaprotein kinase C

Taglines: Phosphorylate a wide variety of protein targets

NCBI Gene ID #: 18049

NCBI Gene Symbol: NGF

Gene Source: N/A

Accession #: P01139

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of PKC was determined to be 512 nmol /min/mg as per activity assay protocol.

Biological activity: This NGF- is fully biologically active when compared to standards. The ED as measured by the dose-dependent induction of human TF-1 cell proliferation is typically 0.1–1 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 105.0 kDa

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml containing 1% HSA or BSA. The solution can be diluted into other aqueous buffers and stored at 4°C for 2-7 days or store at –20°C for future use.

Amino acid sequence: N/A