Description
PKC is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. In the brain, PKC is translocated to cell membranes during ischemia and is rapidly removed or degraded during the second otherwise lethal ischemic insult in preconditioned brains. This suggest that ischemic preconditioning enhances downregulation of cell signaling mediated by PKC and may thereby provide neuroprotection (1).
7764 | PKC gamma Active DataSheet
Biomolecule/Target: N/A
Synonyms: PKC, protein kinase C, gammaprotein kinase C
Alternates names: PKC, protein kinase C, gammaprotein kinase C
Taglines: Phosphorylate a wide variety of protein targets
NCBI Gene ID #: 18049
NCBI Gene Symbol: NGF
Gene Source: N/A
Accession #: P01139
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): The specific activity of PKC was determined to be 512 nmol /min/mg as per activity assay protocol.
Biological activity: This NGF- is fully biologically active when compared to standards. The ED as measured by the dose-dependent induction of human TF-1 cell proliferation is typically 0.11 ng/ml
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 105.0 kDa
Concentration: 0.1mg/ml
Appearance: Liquid
Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml containing 1% HSA or BSA. The solution can be diluted into other aqueous buffers and stored at 4°C for 2-7 days or store at 20°C for future use.
Amino acid sequence: N/A