Description
PKC eta is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKC is predominantly expressed in squamous cell epithelia and induces terminal differentiation of keratinocytes. PKC that is endogenously expressed or overexpressed is found to associate with the cyclin E/cdk2/p21 complex in keratinocytes of mice and humans (1).
7731 | PKCeta Active DataSheet
Biomolecule/Target: N/A
Synonyms: PKC, PKC, Protein kinase C eta
Alternates names: PKC, PKC, Protein kinase C eta
Taglines: Phosphorylate a wide variety of protein targets
NCBI Gene ID #: 4908
NCBI Gene Symbol: NTF3
Gene Source: N/A
Accession #: P20783
Recombinant: Yes
Source: Baculovirus (Sf9 insect cells)
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): 86 nmol phosphate incorporated into peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 103.0 kDa
Concentration: 5µg/50 µl
Appearance: Liquid
Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml.
Amino acid sequence: N/A