PKCeta, Active | 7731

PKC eta is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKC is predominantly expressed in squamous cell epithelia and induces terminal differentiation of keratinocytes. PKC that is endogenously expressed or overexpressed is found to associate with the cyclin E/cdk2/p21 complex in keratinocytes of mice and humans (1).

7731 | PKCeta Active DataSheet

Biomolecule/Target: N/A

Synonyms: PKC, PKC, Protein kinase C eta

Alternates names: PKC, PKC, Protein kinase C eta

Taglines: Phosphorylate a wide variety of protein targets

NCBI Gene ID #: 4908

NCBI Gene Symbol: NTF3

Gene Source: N/A

Accession #: P20783

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 86 nmol phosphate incorporated into peptide substrate (ERMRPRKRQGSVRRRV) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 103.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml.

Amino acid sequence: N/A