PRMT1, human recombinant (Active) | 4865

PRMT1 methylate’s (mono & asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain (may methylate HNRNPA1 and histones) methylate’s SUPT5H. The PRMT1 protein functions as a histone methyltransferase specific for H4. PRMT1 is an essential factor in oncogenesis and is a potential novel therapeutic target in cancer. PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. CAF1 is a new regulator of PRMT1-dependent arginine methylation. PRMT1 arginine-methylate’s MRE11 therefore it regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response. PRMT1 plays a post-translationally part in regulating the transcriptional activity. PRMT1 is found predominantly in the cytoplasm, though a fraction of PRMT1 is located in the nucleus. PRMT1 Human Recombinant (a.a. 1-353) fused with His-MBP tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 750 amino acids and having a molecular mass of 84 kDa. The PRMT1 is purified by proprietary chromatographic techniques.

4865 | PRMT1 human recombinant Active DataSheet

Biomolecule/Target: N/A

Synonyms: HMT2, HRMT1L2, IR1B4

Alternates names: Activation-induced TNFR member Ligand, TNFSF18, GITRL, TL-6

Taglines: A histone methyltransferase specific for H4

NCBI Gene ID #: 8995

NCBI Gene Symbol: TNFSF18

Gene Source: Human

Accession #: Q9UNG2

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Note: Results may vary with PBMC donors.

Biological activity: Determined by its ability to stimulate IL-8 production by human PBMC using a concentration range of 1.0-10.0 ng/ml. Note: Results may vary with PBMC donors.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 14.4 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Tris, pH 8.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: METAKEPCMA KFGPLPSKWQ MASSEPPCVN KVSDWKLEIL QNGLYLIYGQ VAPNANYNDV APFEVRLYKN KDMIQTLTNK SKIQNVGGTY ELHVGDTIDL IFNSEHQVLK NNTYWGIILL ANPQFIS