26
Procathepsin K, mouse recombinant | 1027
- SKU:
- 26-1027-GEN
- Availability:
- Usually shipped in 5 working days
Description
Cathepsin K is a member of the papain cysteine proteinase family and has been identified as the predominant proteinase responsible for the resorption of the bone matrix. The enzyme cleaves proteins such as collagen type I, collagen type II and osteonectin and therefore plays a role in bone remodeling and resorption in diseases such as osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.
1027 | Procathepsin K mouse recombinant DataSheet
Biomolecule/Target: Procathepsin K
Synonyms: Cathepsin O, Cathepsin O2, Cathepsin X, CTSK, CTSO, CTSO2
Alternates names: ANGPT-2
Taglines: Predominant proteinase responsible for the resorption of the bone matrix.
NCBI Gene ID #: 285
NCBI Gene Symbol: ANGPT2
Gene Source: Human
Accession #: O15123
Recombinant: Yes
Source: CHO cells
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2 g/ml.
Biological activity: Determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2 g/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 60-70 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 8.0.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQQNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE LQLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHIIQLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QHDLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTNGI YTLTFPNSTE EIKAYCDMEA GGGGWTIIQR REDGSVDFQR TWKEYKVGFG NPSGEYWLGN EFVSQLTNQQ RYVLKIHLKD WEGNEAYSLY EHFYLSSEEL NYRIHLKGLT GTAGKISSIS QPGNDFSTKD GDNDKCICKC SQMLTGGWWF DACGPSNLNG MYYPQRQNTN KFNGIKWYYW KGSGYSLKAT TMMIRPADFH HHHHH