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QuantiChrom™ beta-Glucosidase Assay Kit

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SKU:
65-DBGD-100-GEN
Availability:
In Stock
€2,665.20

Description

QuantiChrom™ beta-Glucosidase Assay Kit

 

Application: For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism.

Key Features:

  • High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L.
  • Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.
  • Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Method: OD405nm

Samples: Biological

Species: All

Size: 100 tests

Detection Limit: 2 U/L

Shelf Life: 6 months

More Details:

β-GLUCOSIDASE is a glucosidase enzyme which acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose.

Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery.

Gentaur Genprice QuantiChrom™ β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

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Additional Information

Application:
Quantitative determination of β-glucosidase activity by colorimetric (405nm) method.
Procedure:
20 min
Size:
100 Tests
Detection limit:
2 U/L
Shelf life:
6 months
Shipping:
Ambient temperature
Storage:
-20°C.
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