65

QuantiChrom™ Isocitrate Dehydrogenase Assay Kit

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SKU:
65-DIDH-100-GEN
Availability:
In Stock
£1,608.80

Description

QuantiChrom™ Isocitrate Dehydrogenase Assay Kit

 

Application: For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism.

Key Features:

  • Fast and sensitive. Linear detection range (20 μL sample): 0.1 to 100 U/L for 30 min reaction.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method: OD565nm

Samples: Plasma, serum,tissue and culture media etc

Species: All

Size: 100 tests

Detection Limit: 0.1 U/L

Shelf Life: 6 months

More Details:

ISOCITRATE DEHYDROGENASE (IDH) is an enzyme which catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms.

Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. Gentaur Genprice' non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

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Additional Information

Application:
Quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism
Method:
OD565nm
Samples:
plasma, serum,tissue and culture media etc
Species:
all
Procedure:
30 min.Size= 100 tests
Storage:
-20°C
Shipping:
Room Temperature
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1 Review

  • 5
    recommendable

    Posted by Camden on 30th Sep 2022

    This is a highly recommendable company, as my experience with Gentaur has been excellent and all the products are high quality.

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