Recombinant Human IL-2 Protein [E. coli] | 200-009/200-010

Recombinant Human IL-2 Protein [E. coli] | 200-009/200-010 | Gentaur UK, US & Europe Distribution

Species: Human

Host / biotech: E. coli

Comment: N/A

Label: N/A

Clone / Antibody feature: N/A

Subcategory: Cytokines & Growth Factors

Category: Recombinant Protein

Synonyms: IL2; IL-2; TCGF; lymphokine

Isotype: N/A

Application: N/A

Detection Range: The ED50 as determined by the dose-dependent stimulation of proliferation of murine D10G4.1 cells is in the range of 0.1-0.5 ng/ml.

Species Reactivity/Cross reactivity: Human

Antigen: N/A

Description: IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant human IL-2 (modified at the C-terminal end) is a 15.5 kDa protein, containing 156 amino acid residues including one intrachain disulfide bond.

Purity Confirmation: > 98% by SDS-PAGE

Endotoxin: < 0.1 ng per µg of IL-2

Formulation: lyophilized

Storage Handling Stability: The lyophilized IL-2, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted IL-2 should be stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.

Reconstituation: The lyophilized IL-2 should be reconstituted in 50mM acetic acid to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.

Molecular Weight: 15.5 kDa

Lenght (aa): 156

Protein Sequence: MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLHYKDPAANKARKEAELAAATAEQ

NCBI Gene ID: 3558