Retro-CAG-eGFP-VSVG was packaged in HEK293T cells with Ca₃(PO₄)₂ transfection of 3 plasmids of pRetro-CAGGFP, pRetro-RC, and pCMV-VSVG. The pRetro-CAGGFP was constructed by using the components of Moloney Murine Leukemia virus (MoMLV), a combination of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter (CAG), and enhanced green fluorescent protein (eGFP) expression cassette. RetroCAG-eGFP-VSVG is a replication deficient virus and has potentials to infect many types of cell and tissue. It belongs to biosafety level-1 (BSL-1). Retro-CAG-eGFP-VSVG is provided as concentrated viral supernatant at a titer around 107 IU/ml in HEK293T cells.

Feature :

• Bright GFP expression: The Retro-CAG-eGFP-VSVG can express the green fluorescent protein brightly in many cultured cells and animals.
• Replication deficient virus: The Retro-CAG-eGFPVSVG is replication deficient virus.

Applications :

• Virology: The Retro-CAG-eGFP-VSVG can be used as a control virus in virological researches.
• Gene therapy research: The Retro-CAG-eGFP-VSVG can be used as a control retroviral vector when the targeted gene expressed by using retroviral vector.
• Cell biology: The Retro-CAG-eGFP-VSVG can be used for establishing stable GFP expression cell lines.

Storage Conditions :

• The product is shipped on dry ice. Store at -80°C.