SAG12 | Senescence-specific cysteine protease SAG12 | AS14 2771

SAG12 | Senescence-specific cysteine protease SAG12 | AS14 2771 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana SAG12 protein sequence, UniProt:Q38886, TAIR: AT5G45890

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS16 3110 | Anti-Cp2 | Cysteine protease, rabbit antibodiescollection of antibodies involved in senescenceAlgal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 2000 (WB)

Molecular weight: 38 | 28 kDa

Confirmed Reactivity: Arabidopsis thaliana, Brassica napus, Hordeum vulgare

Predicted Reactivity: Actinidia deliciosa, Brassica rapa, Gossypium hirsutum, Petunia hybrida, Morus notabilis, Nicotiana tabacum, Petunia hybrida, Populus trichocarpa, Ricinus communis, Theobroma cacao Species of your interest not listed? Contact us

Not reactive in: Citrus sinensis

Additional Information: Recommended plant extraction buffer: See Durian et al. (2019) . Denature in Laemmli-sample buffer (final concentrations: 5% (v/v) beta-mercaptoethanol, 69 mM Tris-HCl pH 6.8, 11.1% (v/v) glycerol, 2.15 % (v/v) SDS) at 70°C for 5 min

Background: Senescence-specific cysteine protease (SAG12) is a cysteine protease influenced by cytokinin, auxin and sugars and involved in developmental senescence specific cell death.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G45890

Category: Senescence/Cell death

Research Area: Cell Signaling, Apoptosis