sCD27 Ligand, Human Recombinant | 7125

CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. It is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. ’s recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag.

7125 | sCD27 Ligand Human Recombinant DataSheet

Biomolecule/Target: sCD27

Synonyms: TNFSF7, CD70

Alternates names: Growth/Differentiation Factor-2, BMP-9

Taglines: Regulate the immune response by promoting T-cell expansion and differentiation

NCBI Gene ID #: 2658

NCBI Gene Symbol: GDF2

Gene Source: Human

Accession #: Q9UK05

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.

Biological activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 24.1 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: SAGAGSHCQK TSLRVNFEDI GWDSWIIAPKE YEAYECKGGC FFPLADDVTPTK HAIVQTLVHL KFPTKVGKAC CVPTKLSPIS VLYKDDMGVP TLKYHYEGMS VAECGCR