SCF, murine recombinant | 4328

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures. Recombinant murine SCF is an 18.3 kDa protein containing 164 amino acid residues.

4328 | SCF murine recombinant DataSheet

Biomolecule/Target: SCF

Synonyms: Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Alternates names: Glial-Derived Neurotrophic Factor, ATF-1

Taglines: A cytokine playing an important role in hematopoiesis, spermatogenesis, & melanogenesis

NCBI Gene ID #: 14573

NCBI Gene Symbol: GDNF

Gene Source: Human

Accession #: P48540

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): The ED50 was determined by the proliferation of rat C6 cells is 0.2 ng/ml, corresponding to a specific activity of 5 x 10^6 units/mg.

Biological activity: The ED50 was determined by the proliferation of rat C6 cells is 0.2 ng/ml, corresponding to a specific activity of 5 x 10^6 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15.1 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium citrate.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI