SMAD3 Antibody| F43048-0.08ML/F43048-0.4ML/R32172/V9231-20UG

SMAD3 Antibody| F43048-0.08ML/F43048-0.4ML/R32172/V9231-20UG | Gentaur UK, US & Europe Distribution

Background: SMAD3 is a receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: Mouse, Rat, Pig, Chicken

Application: WB, IF, IHC, ELISA

Application Details: Western blot: 1:1000, Immunofluorescence: 1:10-1:50, IHC (Paraffin): 1:10-1:50

Application Note: Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This SMAD3 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 127-156 from the human protein was used as the immunogen for this SMAD3 antibody.

Storage: Aliquot the SMAD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A