SMURF2 Antibody | 7773

SMURF2 Antibody | 7773 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.
The immunogen is located within amino acids 140 - 190 of SMURF2.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.

Positive Control 1: Cat. No. 1285 - C2C12 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 82 kDa
Observed: 85 kDa

Validation: N/A

Isoform: N/A

Purification: SMURF2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: SMURF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, hSMURF2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1) . SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1, 2) . SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3) . Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3) . SMURF2 associates constitutively with SMAD7 (4) .