Description
SMURF2 Antibody | 7773 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.
The immunogen is located within amino acids 140 - 190 of SMURF2.
Research Area: Signal Transduction
Tested Application: E, WB, IHC-P, IF
Application: SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificiy: SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.
Positive Control 1: Cat. No. 1285 - C2C12 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 82 kDa
Observed: 85 kDa
Validation: N/A
Isoform: N/A
Purification: SMURF2 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: SMURF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, hSMURF2
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1) . SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1, 2) . SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3) . Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3) . SMURF2 associates constitutively with SMAD7 (4) .