SPDP Crosslinker | 2328

KEY FEATURES: • SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups) • SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed • Contains a disulfide linkage in its spacer arm with a length of 6.8 Å • The spacer is cleavable with reducing agent, such as DTT, TCEP, etc. APPLICATIONS: • Reversible crosslinking of proteins • N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains • Useful for adding sulfhydryl groups to proteins by attachment to lysine residues • Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates

SPDP Crosslinker is a cleavable heterobifunctional crosslinker.

2328 | SPDP Crosslinker DataSheet

Alternate Name/Synonyms: N-succinimidyl-3-(2-pyridyldithio)propionate; 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester; 3-(2-Pyridyldithio)propionic acid NHS ester

Appearance: White to Off-white Solid

Formulation: N/A

CAS Number: 68181-17-9

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₂H₁₂N₂O₁₀

Molecular Weight: 312.36

Cell-Permeable?: No

Purity: ≥90% by NMR

Solubilities: DMSO or DMF

Handling: Protect from moisture

Country of Origin: USA

Tag Line: A cleavable heterobifunctional crosslinker.

MDL Number: MFCD00009636

PubChem CID: 100682

SMILES: C1CC(=O)N(C1=O)OC(=O)CCSSC2=CC=CC=N2

InChi: InChI=1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2

InChi Key: JWDFQMWEFLOOED-UHFFFAOYSA-N