Sulfo-LC-SPDP | 2325

KEY FEATURES: • Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed • Amine-reactive; the "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 Å spacer arm • More water-soluble than the corresponding LC-SPDP • Membrane-impermeable which enables crosslinking on cell surface • The cleavable spacer arm enables separation of cross-linked products APPLICATIONS: • Reversible crosslinking of proteins • N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains • Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.

Sulfo-LC-SPDP is a water-soluble, amine and thiol (sulfhydryl) reactive heterobifunctional crosslinking reagent.

2325 | Sulfo-LC-SPDP DataSheet

Alternate Name/Synonyms: Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate

Appearance: White to Off-white Solid

Formulation: N/A

CAS Number: N/A

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₈H₂₂O₈N₃S₃Na

Molecular Weight: 527.57

Cell-Permeable?: No

Purity: ≥90% by NMR

Solubilities: H₂O (~ 6 mg/ml)

Handling: Protect from moisture

Country of Origin: USA

Tag Line: A water-soluble, amine and thiol (sulfhydryl) reactive heterobifunctional crosslinking reagent.

MDL Number: N/A

PubChem CID: N/A

SMILES: O=C(CC1S(=O)(O[Na])=O)N(OC(CCCCCNC(CCSSC2=CN=CC=C2)=O)=O)C1=O

InChi: InChI=1S/C18H23N3O8S3.Na/c22-15(7-10-30-31-13-5-4-8-19-12-13)20-9-3-1-2-6-17(24)29-21-16(23)11-14(18(21)25)32(26,27)28;/h4-5,8,12,14H,1-3,6-7,9-11H2,(H,20,22)(H,26,27,28);/q;+1/p-1

InChi Key: JYGUAOMOEQATKJ-UHFFFAOYSA-M