Stable GS-HepG2 Cell Line | T6015

Stable GS-HepG2 Cell Line | T6015

Although, the urea cycle is responsible for most ammonia removal in the liver, glutamine synthetase is an important secondary metabolic pathway also responsible for its removal. The HepG2 cell line has been transformed with a vector containing a glutamine synthetase gene which allows this cell line to metabolism ammonia. With its high glutamine synthetase activity, the HepG2-GS cell line has the ability to metabolize ammonia from its culture media similar to primary hepatocytes. Ammonia-metabolizing cell lines have been used for research into hybrid bioartificial liver support systems, an innovative therapy that has potential to serve as a treatment for liver failure. Therefore, this immortalized cell line may serve as an ideal model for research pertaining to ammonia metabolism systems for long term use in place of primary hepatocytes.

Biosafety:

II

Organism:

Human

Source Organ:

Liver

Growth Properties:

Adherent

Morphology:

N/A

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

male

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow X medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C