Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293, and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™ is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.

Key Features for Trans-HiTM

• Excellent transfection for single DNA transfection or multi DNA co-transfection
• Rapid onset and long-lasting transfection
• Suitable for very long DNAs
• Simple and easy to use transfection procedure
• Robust and reproducible transfection
• High level of recombinant protein production
• Very cost-efficient
• Long shelf-life: 12 months

Specifications:

Product type: In vitro cell transfection reagent
Applications: Plasmid transfection, protein overexpression
Technique: Polymer-based DNA nanoparticles
Cell type: Established cell lines, primary cells, hard-to-transfect cells Adherent cells, and suspension cells
Sample type: Plasmid DNA
Serum compatibility: Yes
Product size: 0.5, 1.0 and 10×1.0 mL
Number of transfections: Up to 660 in 24-well plates with 1.0mL reagent up to 330 transfections in 6-well plates
Shelf-life: 12 months or longer when store refrigerated (2-8°C)
Shipping condition: Room temperature

Comparison study of In-vitro plasmid DNA transfection of Trans-Hi^TM and Lipofectamine® 2000 in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per well used for both products in 24 well plates 30 hours post-transfection.

Trans-Hi™/DNA ratio (µL/µg) (HEK293T)

Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal transfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in 24 well plates 30 hours post-transfection, 0.5ug DNA per well).

Head-to-head comparison Trans-Hi™ against Lipofectamine 2000 HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post-transfection).

Comparison study of In-vitro plasmid DNA transfection of Trans-Hi™ and Lipofectamine® 2000 in Hela cells with GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post-transfection).