The Tribo™ Human Carcinoembryonic Antigen ELISA Kit is designed for in vitro quantitative determination of CarcinoEmbryonic Antigen (CEA) concentrations in human serum and plasma samples. This assay employs an antibody specific for Human Carcino Embryonic Antigen CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and Carcino Embryonic Antigen CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Carcino Embryonic Antigen CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Carcino Embryonic Antigen CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The concentration of CEA in the samples is calculated opposite to the standard curve.

Applications:

Direct Assays: quantitative CEA in different tissues.

Key Features:

• Sensitivity: The minimum detectable dose of human CEA is typically less than 2.0 pg/mL.
• Specificity: This assay recognizes recombinant and natural human CEA. No cross-reactivity with any other cytokine is observed.

Kit Contents:

Storage Conditions:

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receipt.