26
Turbo3C (HRV3C) Protease, Recombinant | 9206
- SKU:
- 26-9206-GEN
- Availability:
- Usually shipped in 5 working days
Description
s Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly (independent of Pro). The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C with high specific activity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. This HRV3C Protease is a 47 kDa protein with both GST and His tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.
9206 | Turbo3C HRV3C Protease Recombinant DataSheet
Biomolecule/Target: N/A
Synonyms: HRV3C Protease
Alternates names: Protease
Taglines: A restriction grade protease which cleaves at the PreScission Site
NCBI Gene ID #: 7173
NCBI Gene Symbol: TPO
Gene Source: N/A
Accession #: P07202
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found <1 ng/ml, corresponding to a specific activity of >1 x 10 IU/mg.
Results: N/A
Binding Capacity: N/A
Unit Definition: > 1 unit/µg. One unit of Turbo3C (HRV3C) Protease cleaves >95% of 100 µg of target protein at 4°C for 16 hours
Molecular Weight:
Concentration: N/A
Appearance: Liquid
Physical form description: 2 mg/ml in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP, and 50% glycerol
Reconstitution Instructions: Reconstitution in purified HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).
Amino acid sequence: N/A